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Zn-induced precipitation. (a,c) zinc-free human recombinant insulin (5 mg/mL) and Mo-LPI (5 mg/mL) solutions in 0.05 M Tris-HCl, pH 7.5, respectively; (b,d) zinc-free human recombinant insulin and Mo-LPI solutions after the addition of 1 M zinc chloride, respectively. Arrows indicate the protein precipitate.
