Table 1.
Crowding Agent | KSV, M−1 | A297 | r | [θ]222·10−3, deg·cm2·dmol−1 |
---|---|---|---|---|
PEG-12000 | 2.1 | 1.12 | 0.20 | −11 |
PEG-4000 | 2.2 | 1.16 | 0.18 | −18 |
PEG-600 | 1.7 | 1.18 | 0.18 | −16 |
Ficoll-70 | 1.3 | 0.82 | 0.19 | −16 |
Dextran-70 | 1.7 | 0.77 | 0.17 | −15 |
No crowding agents * | 4.7 | 1 | 0.15 | −14 |
Glucose * | 1.9 | 1 | 0.16 | −15 |
All experiments were performed in buffer solutions (see Materials and Methods). Concentration of all crowding agents was 300 mg/mL. * For comparison, the same experiments were conducted in the absence of crowding agents and in solutions of glucose in concentration of 300 mg/mL.