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. 2016 Dec 28;22(1):36. doi: 10.3390/molecules22010036

Figure 5.

Figure 5

XN decreases the stemness of MCF-7/ADR. (A) XN inhibits the colony formation of MCF-7/ADR cells. Colony formation assay was performed as described in the Materials and Methods section. Shown is a representative from three experiments with similar results. The histogram shows the number of colonies in the presence and absence of XN. * p < 0.05, ** p < 0.01 versus medium control; (B) XN disturbs the preformed colonies of MCF-7/ADR cells. Values represent the means ± SD of triplicate measurements; * p < 0.05, ** p < 0.01 versus medium control; (C) XN inhibits the migration of MCF-7/ADR. Cells were treated with XN (0–10 μM) for 48 h, and analyzed with scratch-wound assay. Dotted lines show the migration edge of the cells. Histogram shows the relative migration distance in the presence and absence of XN. * p < 0.05, ** p < 0.01 versus medium control; (D) XN inhibits mammospheres formation in vitro. MCF-7 cells were cultured in ultra-low-attachment plates with serum-free medium and treated with XN for 25 days. Mammospheres were observed and photographed under microscope (×200, scale bar = 500 μm).