Table 1.

Kinetic constants of hQR2 for the substrates tested. Results are the mean ± SD of three separate determinations.

Substrate a	Vmax (µmol·mL−1·min−1)	KM (µM)
Menadione	4264 ± 134	10 ± 1
1	789 ± 39	47 ± 5
8	1471 ± 98	12 ± 2
10	730 ± 59	21 ± 4
12	1255 ± 93	30 ± 6

^a All substrate stock solutions (20 mM) were prepared in 100% DMSO and then diluted in Tris-β-octyl buffer (50 mM Tris-HCl, 1 mM octyl-GP) pH 8.5 (DMSO final 5.5%) and reactions are followed at 37 °C.