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. 2017 Jan 2;22(1):74. doi: 10.3390/molecules22010074

Table 1.

Primers used in this study. ORF: open-reading frame.

Usage Primer Name Primer Sequence 5′-3′
ORF-PCR GbAACT-F ATGGGTTCTACATCAGGCTCAGATTTGTT
GbAACT-R TTACATGAGCTCCAAGACAACTGCAG
GbMVK-F ATATTGGGAGTTCAGAATGGTGC
GbMVK-R ACATTTATGAGCTTGAGCATTGGAA
qRT-PCR GbAACT-qF CATTGGGGGTCTGAGTGGTTC
GbAACT-qR CATTGTTGCTTTCATTCCCGA
GbMVK-qF GGCATTAGTTGCTGGAGTTTCTGAG
GbMVK-qR GGTGCCTGAATAATCTCTGCCATCT
GbGAPDHF CTGGCGTAGAGTATGTGGTTGAAT
GbGAPDHR CACGCCAACAACGAACATG
Yeast-PCR GbAACT-yup CGGGATCCATGGGTTCTACATCAGGCTCAG
GbAACT-yud CGGAATTCTTACATGAGCTCCAAGACAACTG
GbMVK-yup CGGGATCCATATTGGGAGTTCAGAATGGTGC
GbMVK-yud CGGAATTCACATTTATGAGCTTGAGCATTGGAA