Figure 1.
Aspalathin (ASP) increased the expression of Nrf2 in H9c2 cardiomyocytes. (A) The degree of Nrf2 knockdown in H9c2 cardiomyocytes after transfection with either small interfering RNA (siNrf2) or scrambled RNA (scrRNA) for 24 h; (B) H9c2 cardiomyocytes transfected with siNrf2 or scrRNA for 24 h were exposed to 33 mM glucose (HG) for 24 h, followed by treatment with 1 µM ASP for 6 h. Results are expressed as the mean ± SEM of three independent biological experiments relative to the normal glucose (NG) control, each done in triplicate. ** p ≤ 0.001, *** p ≤ 0.0001 vs. NG, # p ≤ 0.05 vs. HG, ††† p ≤ 0.0001 vs. scrRNA + ASP.