Figure 3.

Effects of elderflower extracts, constituents and metabolites on oleic acid uptake in human myotubes. Myotubes were treated with (A) 12.5, 25 and 50 µg/mL of different crude extracts; (B,C) 0.1, 1 and 10 µM of elderflower phenolic compounds and metabolites for 2 days. Thereafter, the cells were exposed to [¹⁴C]-oleic acid (37 kBq, 100 µM) for 4 h as described in Materials and Methods. A synthetic liver X receptor (LXR) agonist T0901317 (10 μM) was used as positive control. The figures show [¹⁴C]-oleic acid uptake given as means ± SEM (n = 3) from separate experiments. * p < 0.05 vs. control (0.1% DMSO).