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Anticancer activity studies. The MTT assays of MDA-MB-231 (A) and MCF-7 cells (B) were treated with copper, ICA, and ICA-Cu for 24 h at various concentrations, as indicated. 1% DMSO was used as a control. ** p < 0.01 and *** p < 0.001, compared to control. The means ± SD of three independent experiments are indicated by the bars; (C) the microscopy photographs of MDA-MB-231 and MCF-7 cells treated with ICA-Cu for 24 h, by a magnification of ×400.
