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. 2016 Dec 28;22(1):37. doi: 10.3390/molecules22010037

Figure 3.

Figure 3

Effects of VEE on activation of the FcεRI and arachidonate cascades. RBL-2H3 cells were seeded in a 6-well plate (5 × 105 cells/well) in MEM-α with 10% FBS at 37 °C overnight and further incubated with DNP-IgE for 24 h. IgE-sensitized RBL-2H3 cells were preincubated with VEE for 1 h and then stimulated with antigen for 4 h or 10 min. The cells were washed with 1× DPBS and lysed with a cell lysis buffer. The expression of p-cPLA2, COX-2, p-5-LO, p-Syk, p-Fyn, p-Lyn, p-PLCγ1, p-PLCγ2, p-PKCδ, p-Akt, p-JNK, p-ERK, p-p38, or β-actin was determined as described in the Materials and Methods section. Similar results were obtained in three independent experiments. ** p < 0.01 versus DNP-HSA-treated group. (A) p-cPLA2, p-5-LO, and COX-2; (B) p-Syk, p-Fyn, and p-Lyn; (C) p-PLCγ1/2 and p-PKCδ; (D) p-Akt, p-JNK, p-ERK, and p-p38.