Table 1.

Protein tyrosine phosphatase 1B and α-glucosidase inhibitory activities of the MeOH extract of Cassia obtusifolia and its solvent soluble fractions.

Test Samples	PTP1B a	α-Glucosidase b
	IC50 (Mean ± SEM)	IC50 (Mean ± SEM)
MeOH extract	14.79 ± 0.31	200.07 ± 7.90
CH2Cl2 fraction	85.31 ± 3.43	359.36 ± 10.81
EtOAc fraction	57.90 ± 0.92	74.50 ± 4.93
n-BuOH fraction	172.82 ± 4.87	372.12 ± 11.88
H2O fraction	214.52 ± 3.42	434.02 ± 12.61
Ursolic acid c	3.37 ± 0.18
Acarbose d		123.54 ± 0.29

^a,b Final concentration of test samples and positive controls were 100 µg/mL (for PTP1B) and 400 µg/mL (for α-glucosidase), dissolved in 10% DMSO: 50% inhibition concentrations (IC₅₀, µg/mL) are expressed as the mean ± SEM of triplicate experiments. ^c,d Ursolic acid and acarbose were used as positive controls for the PTP1B and α-glucosidase assays, respectively.