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. 2016 Dec 29;22(1):44. doi: 10.3390/molecules22010044

Figure 2.

Figure 2

Isolation and identification of BaCHS. (A) RT-PCR products from the petals of Brunfelsia acuminata with primers CHS-ConF /CHS-ConR for lane 1. Marker: DL-2000 marker; (B) 3′RACE-PCR products from the petals of Brunfelsia acuminata with GSP1/ GSP2 3′RACE-PCR inner primer; (C) 5′RACE-PCR products from the petals of Brunfelsia acuminata with GSP3/GSP4 5′RACE-PCR inner primer; (D) The full cDNA products of Brunfelsia acuminata petals with FPF/FPR primers; (E) RT-PCR and RACE-PCR product lengths with different primers.