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. 2016 Dec 29;22(1):44. doi: 10.3390/molecules22010044

Table 1.

Primers used in the study of BaCHS and RT-qPCR.

Primer Primer Sequence Use
CHS-conF(forward) 5′-GAGAARTTCMRGCGCATGTGYG-3′ cDNA
CHS-conR(reverse) 5′-GCTTGGTGAGYTGRTAGTCRG-3′
GSP1(forward) 5′-CGGCGCATGTGTGATAAATCA-3′ 3′RACE-PCR
GSP2(forward) 5′-GGTACATGCACTTAACAGAGG-3′
UPM 5′-CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT-3′
GSP3(reverse) 5′-TCACAAATCTTGGGGTTCTC-3′ 5′RACE-PCR
GSP4(reverse) 5′-CTAGCATCAAGGGAAGGAGCC-3′
UPM 5′-CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT-3′
FPF(forward) 5′-ATGGTGACCGTAGAGGAGTATCG-3′ Full-length
FPR (reverse) 5′-GAACCCACTAAGTAGCCACACTG-3′
CHS-F(forward) 5′-CAAAGGAGGGCTTAGGAACTACT-3′ RT-qPCR
CHS-R(reverse) 5′-CAAATTCAACCCCAAGAACAAATGA-3′
BaActin-F(forward) 5′-AACCATAAACGATNCCGACCAG-3′
BaActin-R(reverse) 5′-NCTTGCGACCATACTCCC-3′