Table 1.
Catalytic properties of the biocatalysts prepared by immobilization of PFL and TLL on mono and heterofunctional silica particles.
| Protein Loading = 1 mg/g of Support | ||||||
| Support | TLL | PFL | ||||
| IY d (%) | RA e (%) | HA f (IU/g) | IY d (%) | RA e (%) | HA f (IU/g) | |
| OS a | 88.6 ± 1.0 | 110.4 ± 0.4 | 104.7 ± 0.3 | 92.0 ± 1.8 | 208.1 ± 4.1 | 20.3 ± 0.7 |
| OSGlu a | 89.2 ± 1.6 | 36.9 ± 1.3 | 35.2 ± 1.1 | 87.5 ± 0.6 | 110.3 ± 5.7 | 9.8 ± 0.5 |
| OSEpx b | 84.3 ± 0.1 | 58.6 ± 1.3 | 75.5 ± 0.8 | 80.4 ± 3.6 | 157.1 ± 7.0 | 13.4 ± 0.9 |
| OSGlx c | 86.9 ± 0.4 | 50.5 ± 3.5 | 43.8 ± 2.6 | 90.0 ± 1.5 | 139.7 ± 2.4 | 13.3 ± 1.1 |
| Protein Loading = 10 mg/g of Support | ||||||
| Support | TLL | PFL | ||||
| IY d (%) | RA e (%) | HA f (IU/g) | IY d (%) | RA e (%) | HA f (IU/g) | |
| OS a | 96.9 ± 0.3 | 93.3 ± 0.1 | 169.1 ± 9.2 | 89.6 ± 0.5 | 55.2 ± 0.1 | 132.7 ± 2.1 |
| OSGlu a | 94.9 ± 0.2 | 47.6 ± 0.1 | 84.6 ± 0.6 | 90.3 ± 1.1 | 39.8 ± 0.1 | 96.4 ± 0.6 |
| OSEpx b | 96.7 ± 0.8 | 56.1 ± 0.1 | 101.5 ± 1.6 | 88.0 ± 0.9 | 5.3 ± 1.2 | 12.6 ± 0.2 |
| OSGlx c | 95.5 ± 0.6 | 33.9 ± 0.2 | 60.7 ± 1.6 | 90.3 ± 1.4 | 6.0 ± 1.0 | 14.6 ± 0.2 |
a 25 °C, pH 7.0 (10 mM sodium phosphate buffer) for 6 h. b 25 °C, pH 7.0 (10 mM sodium phosphate buffer), followed by incubating at pH 10.0 (100 mM sodium carbonate buffer) for 3 h—Remaining epoxy groups were blocked with 3 M glycine solution (25 °C for 1 h). c 25 °C, pH 7.0 (10 mM sodium phosphate buffer), followed by incubating at pH 10.0 (100 mM sodium carbonate buffer) for 3 h—Remaining free aldehydes and Schiff’s bases were reduced with sodium borohydride (1 mg/mL, 25 °C for 1 h). d Immobilization yield. e Recovered activity. f Apparent hydrolytic activity.