Determining the size of a nanoparticle from fluorescence anisotropy decay curves as measured by TCSPC. (A) Exemplary anisotropy decay for CMS/NR. The slowest rotational correlation time reflecting the tumbling of the whole nanocarrier is indicated; (B) Plot of obtained for CMS/NR against . The volume of the rotating molecule, here CMS, can be obtained from the slope of a linear fit to the data and hence the diameter and radius can be calculated; (C) Diameters of dPGS-ICC calculated directly from as a function of temperature. A shrinkage in size can clearly be seen. (A,B, adapted with permission from Boreham et al. [61]. © 2014 The American Chemical Society; C, adapted from Boreham et al. [15] by permission of John Wiley and Sons © 2014).