Figure 10.

Fluorescence lifetime microscopy (FLIM) of CMS–ICC nanotransporters. CMS–ICC nanotransporters were applied onto normal and stripped human skin for 24 h. Representative overlay images (FLIM and bright field) of the same area are shown: (A), As a control, an aqueous ICC solution was applied to normal skin. CMS-ICC nanotransporters were applied to (B), normal skin and (C), stripped skin. The false color coding is based on the unique fluorescence lifetimes of CMS–ICC nanotransporters (cyan, red) or ICC (blue) in the different skin layers. (D), the fluorescence lifetime traces (as measured by TCSPC) of CMS–ICC nanotransporters and ICC in aqueous solution are shown. (E–G), The fluorescence lifetime traces of CMS–ICC nanotransporters and ICC are shown in the same colors as used in the corresponding overlay images (A–C). All lifetime traces are normalized to 1. Scale bar: 100 μm (reprinted from Alnasif et al. [11] with permission from Elsevier, © 2014).