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. 2017 Feb 6;22(2):242. doi: 10.3390/molecules22020242

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cearoin increases the intracellular reactive oxygen species (ROS) production and nitric oxide (NO) generation in neuroblastoma SH-SY5Y cells. SH-SY5Y neuroblastoma cells were treated with cearoin in various concentrations (0, 10, 20, 40, 80, or 160 μM) and incubated for 12 h. (A) Intracellular ROS production was measured using DCFDA fluorescence assay. Each bar represents the mean percentage alterations above the control (±SD) (n = 5); (B) total NO release was measured using Griess method. Each bar represents the mean nitrite concentration (±SD) (n = 6). Differences were considered statistically significant at * p < 0.05 and *** p < 0.001 as compared with the control.