Table 1.

Comparison of methods used for in vitro AChEI detection.

Method	Advantages	Disadvantages
Ellman’s method	In vitro testing; no requirement for animal tissues	Large consumption of reagents; difficulty in large-scale preparation of some samples; often nonideal results, inappropriate for high-throughput screens
Modified Ellman’s method	Simplified assay protocol, reduced experimental time, high-throughput screens	Complicated protocol for isolation and purification of samples; interference from organic solvents
TLC bioautography	Reduced steps in sample isolation and purification protocol; elimination of interference from organic solvents	False-positive experimental results
Combined LC-MS/modified Ellman’s method	High specificity and detection sensitivity	Synchronous coupling by the instrument, requirement of additional samplers; false-positive experimental results; expensive materials