Figure 1. Three CpG epigenetic age-predictor for mice.

(a–c) DNA methylation (DNAm) of three CpGs in the genes Prima1, Hsf4 and Kcns1 was analyzed by pyrosequencing in 24 C57BL/6 mice (training set). Coefficient of determination (R²) of DNAm versus chronological age is indicated. (d) Based on these age-associated DNAm changes a multivariable model for age prediction was calculated. (e–g) Subsequently, two independent validation sets were analyzed: 21 C57BL/6 mice from the University of Ulm and 19 C57BL/6 mice from the University of Groningen (validation sets 1 and 2, respectively). (h) Age predictions with the three-CpG-model revealed a high correlation with chronological age in the independent validation sets (MAD = mean absolute deviation; MAE = median absolute error).

Figure 1—figure supplement 1. Target sequences of pyrosequencing assays.

Sequences for the three genomic regions are depicted and CpG sites (red) are numbered by the dispensation order. The relevant CpGs with highest age-correlation are highlighted in bold.