Table 1. Summary of the features of proteins identified in proteomic analysis of the TgApiCox25 complex.
Similarity searches were performed using HMMER (https://www.ebi.ac.uk/Tools/hmmer/). The accession numbers listed were derived from http://EuPathDB.org (apicomplexan and chromerid species) or www.ncbi.nlm.nih.gov (all others). Abbreviations: Plasmodium falciparum (Pf), Cryptosporidium parvum (Cp), Vitrella brassicaformis (Vb), Saccharomyces cerevisiae (Sc), Homo sapiens (Hs), Arabidopsis thaliana (At).
| ToxoDB gene ID (http://toxodb.org) | Protein annotation | Predicted protein mass (kDa) |
Mitochon-drial proteome (this study) |
Phenotype score (Sidik et al., 2016) |
Similarity search (E-value) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Pf | Cp |
Vb
(Chromerid) |
Sc
(Fungi) |
Hs
(Animal) |
At
(Plant) |
|||||
| TGGT1_264040 | Hypothetical protein (TgApiCox25) |
24.5 | ✓ | −2.54 | Conserved unknown protein PF3D7_1464000.1 (2.4e−53) |
x | Hypothetical Protein Vbra_12326 .t1 (5.2e−29) |
x | x | x |
| TGGT1_265370 | Hypothetical protein (TgApiCox16) |
16.0 | ✓ | 1.56 | x | x | x | x | x | x |
| TGGT1_209260 | Putative cytochrome c oxidase subunit (TgCox5b) | 34.8 | ✓ | −3.07 | Putative COX5B PF3D7_0927800.1 (4.3e−101) |
x | COX5B-2 Vbra_9355 .t1 (1.6e−92) |
Cox4p P04037 (0.32) |
Cox5B NP_001853.2 (0.05) |
COX5b At1g80230 (2.1e−08) |
| TGGT1_221510 | Hypothetical protein (TgApiCox18) |
17.9 | ✓ | −3.28 | Conserved unknown protein PF3D7_0523300.1 (1.5e−48) |
x | Hypothetical Protein Vbra_21271 .t1 (5.2e−45) |
x | x | x |
| TGGT1_262640 | Cg8 family protein (TgApiCox23) |
23.8 | ✓ | −3.49 | Cg8 protein PF3D7_0708700.1 (3.1e−64) |
x | Hypothetical Protein Vbra_3012 .t1 (2.4e−53) |
x | x | x |
| TGGT1_297810 | Hypothetical protein (TgApiCox30) |
29.6 | ✓ | −3.64 | Conserved unknown protein PF3D7_0915700.1 (1.2e−46) |
x | Hypothetical Protein Vbra_17445 .t1 (6.7e−33) |
x | x | x |
| TGGT1_247770 | Hypothetical protein (TgApiCox19) |
19.2 | ✓ | −2.61 | Conserved unknown protein PF3D7_1402200.1 (1.2e−34) |
x | Hypothetical Protein Vbra_2065 .t1 (1.7e−27) |
x | x | x |
| TGGT1_229920 | Hypothetical protein (TgApiCox35) |
35.0 | ✓ | −3.84 | Conserved unknown protein PF3D7_0306500.1 (1.5e−90) |
x | Hypothetical Protein Vbra_6819 .t1 (1.6e−73) |
x | x | x |
| TGGT1_306670 | Hypothetical protein (TgApiCox26) |
25.8 | ✓ | −3.68 | Conserved unknown protein PF3D7_1439600.1 (2.6e−43) |
x | Hypothetical Protein Vbra_888 .t1 (1.2e−36) |
x | x | x |
| TGGT1_226590 | Putative cytochrome c oxidase subunit (TgCox2a) | 34.5 | ✓ | −3.80 | Cytochrome c oxidase subunit 2 PF3D7_1361700.1 (4.9e−58) |
x | Cytochrome c oxidase subunit 2 Vbra_8641 .t1 (3.6e−33) |
Cox2 P00410 (2.6e−06) |
Cox2 P00403 (0.0004) |
Cox2 P93285 (3.3e−06) |
| TGGT1_310470 | Putative cytochrome c oxidase subunit (TgCox2b) | 21.2 | ✓ | −4.18 | Cytochrome c oxidase subunit 2 PF3D7_1430900.1 (7.6e−75) |
x | Cytochrome c oxidase subunit 2 Vbra_14923 .t1 (4.2e−7) |
Cox2 P00410 (4.3e−31) |
Cox2 P00403 (9.2e−29) |
Cox2 P93285 (3.8e−37) |
| TGGT1_286530 | Hypothetical protein (TgApiCox24) |
25.4 | ✓ | −2.82 | Conserved unknown protein PF3D7_1362000.1 (6.0e−45) |
x | Hypothetical Protein Vbra_10089 .t1 (1.2e−11) |
x | x | x |
| TGGT1_254030 | Zinc finger CDGSH-type domain-containing protein (TgApiCox13) |
13.2 | ✓ | −4.26 | CDGSH iron-sulfur domain-containing protein PF3D7_1022900.1 (7.8e−42) |
x | CDGSH iron-sulfur domain-containing protein 3 Vbra_4701 .t1 (1.2e−44) |
x | CDGSH iron-sulfur domain-containing protein 3 (2.7e−11) |
x |
| TGGT1_242840 | Membrane protein (TgApiCox14) |
13.9 | ✓ | −3.58 | Conserved unknown protein PF3D7_1339400.1 (4.1e−16) |
x | Hypothetical Protein Vbra_9996 .t1 (1.8e−9) |
x | x | x |
| TGVEG_442760 | Cytochrome C family oxidase subunit III (TgCoxIII) |
16.8 | - | N/A | Cytochrome c oxidase subunit 3 mal_mito_1 (5.2e−5) |
x | x | x | x | x |