Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo
sapiens) |
PCSK9 | NA | Uniprot Q8NBP7 | |
| Gene (Homo
sapiens) |
A1AT | NA | Uniprot P01009 | |
| Gene (Homo
sapiens) |
SURF4 | NA | Uniprot O15260 | |
| Cell line (Homo
sapiens) |
HEK293T | ATCC | RRID:CVCL_1926 | |
| Cell line (Homo
sapiens) |
T-Rex-293 | Invitrogen | RRID:CVCL_D585 | |
| Antibody | anti-PCSK9 (rabbit polyclonal) |
Cayman Chemical | RRID:AB_569536 | (1:1000) |
| Antibody | anti-GFP (rabbit monoclonal) |
Abcam | RRID:AB_303395 | (1:5000) |
| Antibody | anti-mCherry (rabbit polyclonal) |
Abcam | RRID:AB_2571870 | (1:1000) |
| Antibody | anti-GAPDH (rabbit monoclonal) |
Abcam | RRID:AB_2630358 | (1:10,000) |
| Antibody | anti-β-actin (mouse monoclonal) |
Santa Cruz | RRID:AB_2714189 | (1:10,000) |
| Antibody | anti-LMAN1 (rabbit monoclonal) |
Abcam | RRID:AB_10973984 | (1:5000) |
| Antibody | anti-calnexin (rabbit monoclonal) |
Cell Signaling | RRID:AB_2228381 | (1:2000) |
| Antibody | anti-GM130 (rabbit monoclonal) |
Abcam | RRID:AB_880266 | (1:1000) |
| Antibody | anti-BiP (rabbit monoclonal) |
Abcam | RRID:AB_10859806 | (1:5000) |
| Antibody | anti-PDI (rabbit monoclonal) |
Cell Signaling | RRID:AB_2156433 | (1:1000) |
| Antibody | HRP-conjugated anti- FLAG (goat polyclonal) |
Abcam | RRID:AB_299061 | (1:10,000) |
| Antibody | HRP-conjugated anti- mouse secondary (goat polyclonal) |
BioRad | RRID:AB_11125547 | (1:5000) |
| Antibody | HRP-conjugated anti- rabbit IgG (goat polyclonal) |
BioRad | RRID:AB_11125142 | (1:5000) |
| Antibody | FITC-conjugated anti- FLAG (mouse monoclonal) |
Sigma | RRID:AB_439701 | (1:500) |
| Antibody | Alexa647-conjugated anti-rabbit secondary (donkey polyclonal) |
Abcam | ab150075 | (1:500) |
| Recombinant DNA reagent | pLentiCRISPRv2 | Addgene | 52961 | |
| Recombinant DNA reagent | pX459 | Addgene | 62988 | |
| Recombinant DNA reagent | pNLF-C1 | Promega | E1361 | |
| Recombinant DNA reagent | pDEST-pcDNA5-BirA-FLAG-C-term | PMID 24255178 | ||
| Recombinant DNA reagent | pENTR/D-TOPO | Invitrogen | K2400-20 | |
| Chemical compound, drug | brefeldin A | BioLegend | 420601 | |
| Chemical compound, drug | dithiobis(succinimidyl propionate) | Pierce | 22586 |