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. 2018 Sep 12;4(9):eaar3938. doi: 10.1126/sciadv.aar3938

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 3 — Top: IgG IP (IgG), mTOR IP, ETV7 IP, Raptor IP, or Rictor IP of Karpas-299 cell lysate immunoblotted for mTOR, ETV7, Raptor, or Rictor. Bottom: Immunoblot of IgG IP (IgG), mTOR IP (mTORC1/2/3), ETV7 IP (mTORC3), Raptor IP (mTORC1), or Rictor IP (mTORC2) of Karpas-299 lysate incubated with recombinant GST-tagged P70S6K peptide after preincubation with vehicle (−) or rapamycin/FKBP-12 (+) (top blot), vehicle (−) or OSI-27 (+) (middle blot), and vehicle (−) or AZD-8055 (+) (bottom blot) was probed for p-P70S6K^Thr389. GST was used as a loading control.