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. 2018 Sep 19;12:216. doi: 10.3389/fnbeh.2018.00216

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Copyright © 2018 Ali, MacKay, Graitson, James, Cayer, Audet, Kent, Abizaid and Merali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Singly housed animals were assigned to four groups: (1) Chow + Stress; (2) Palatable + Stress; (3) Chow + No Stress and (4) Palatable + No Stress. Groups assigned to the stressor condition were exposed to a different episodic stressor per day from postnatal days (PD) 27–29 while non-stressed animals received daily handling. The following were evaluated in adulthood: Group 1—40 animals (n = 10 per group) were tested for behavior (social interaction), adiposity and gene expression. Group 2—48 animals (n = 12 per group) were tested for hypothalamic-pituitary-adrenal (HPA) activity after an acute stressor (air-puff) and for glucose tolerance.