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. 2018 Sep 19;9:2011. doi: 10.3389/fimmu.2018.02011

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Copyright © 2018 Ulloa-Morales, Goodyear and Silverman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Extent of in vivo SpA-mediated B cell depletion reflects in vitro binding affinity. Assessment of SpA mediated depletion of transgenic T15i (+/−) B-cells following sham pretreatment or pretreatment with human polyclonal IgG (hu pIgG) or human polyclonal IgG F(ab)'2 (pIgG Fab'2) or monoclonal murine IgG, as indicated. At 72 h after SpA (or sham) treatment, levels of residual T15i B cells are indicated for individual mice. Results represent residual splenic IgM^a + B cells, after gating on adoptively transferred CFSE-labeled mononuclear cells, that identified only the B220+ IgM^a+ Cells. After VHIII Fab IgG2a treatment, all mice died acutely () and residual B cells could therefore not be quantitated. Mann-Whitney test, *p < 0.05, ^**p < 0.005, ^***p < 0.001, ^****p < 0.0001. NS, not significant. Binding activity data of murine IgG isotypes were from Wikipedia.

Inline graphic — Extent of in vivo SpA-mediated B cell depletion reflects in vitro binding affinity. Assessment of SpA mediated depletion of transgenic T15i (+/−) B-cells following sham pretreatment or pretreatment with human polyclonal IgG (hu pIgG) or human polyclonal IgG F(ab)'2 (pIgG Fab'2) or monoclonal murine IgG, as indicated. At 72 h after SpA (or sham) treatment, levels of residual T15i B cells are indicated for individual mice. Results represent residual splenic IgM^a + B cells, after gating on adoptively transferred CFSE-labeled mononuclear cells, that identified only the B220+ IgM^a+ Cells. After VHIII Fab IgG2a treatment, all mice died acutely () and residual B cells could therefore not be quantitated. Mann-Whitney test, *p < 0.05, ^**p < 0.005, ^***p < 0.001, ^****p < 0.0001. NS, not significant. Binding activity data of murine IgG isotypes were from Wikipedia.