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. 2018 Sep 19;9:1352. doi: 10.3389/fpls.2018.01352

FIGURE 1.

FIGURE 1

In situ hybridizations of integument regulators to female Amborella flower sections. (A) Atr.INO expression in the growing outer integument at early Stage 7. (B) Atr.INO expression in the near fully grown outer integument at late Stage 7. (C) Atr.INO expression is no longer apparent in the outer integument (LS) in mature flowers. (D) Atr.ATS expression in the inner integument at floral bud Stage 7. (E) Atr.ATS expression in the inner integument persists in the mature flower. (F) Narrow Atr.PHB/PHV expression in the chalaza and strong expression in the adaxial domain of the ovary wall at early Stage 7. (G) Weak Atr.PHB/PHV expression persists in the abaxial domain of the inner integument at late Stage 7. (H) Atr.REV is extensively expressed in the chalaza, and strongly expressed in the adaxial domain of the ovary wall at early Stage 7. (I) Weak Atr.REV expression persists in the adaxial domains of both integuments at late Stage 7. (J) Atr.ARF4 expression in the abaxial domain of both integuments at early Stage 7. (K) Very weak Atr.ETT expression in the abaxial domain of both integuments at early Stage 7. (L) Atr.ARF4 expression is no longer apparent in the integuments in the mature flower. (M) Atr.ETT expression is no longer apparent in the integuments in the mature flower. (N,O) Toluidine-blue stained sections of Stage 7 and mature ovules, respectively, showing anatomical details. Tissues zones in panels (N,O) have been delimited with dotted white lines for clarity. Developmental stages are as defined by Buzgo et al. (2004). abw, abaxial domain of the ovary wall; adw, adaxial domain of the ovary wall; c, chalaza; cp, chalazal pole; f, funiculus; ii, inner integument; mpp, micropyle pole; n, nucellus; and oi, outer integument. Scale bars = 100 μm.