FIG 4.
Genetic dissection of lipogenesis of a high-producing K. marxianus strain, Km6. (A) General overview of lipid-related metabolism. Genes in red were inactivated with CRISPRNHEJ, and genes in green were overexpressed using plasmids. (B) Percentage of fatty acids in dry cell weight (DCW) after 24 h under lipogenic conditions at 42°C for several variants of Km6 (wild type and mutants). (C) Percentage of fatty acids in dry cell weight for several Km6 variants containing ACC1, DGA1, and ACL1/2 overexpression plasmids. In panels B and C, all experiments were carried out in biological triplicate, with mean values and standard deviations shown. Lipogenesis medium in panels B and C contained monosodium glutamate instead of ammonium sulfate.