Figure 3.

TRIM32 Activates the β‐catenin Pathway. A, Western blotting was performed to measure β‐catenin expression in SGC7901 and AGS cells expressing TRIM32 shRNA or MKN28 cells overexpressing TRIM32. B, qRT‐PCR was performed to measure β‐catenin expression in the indicated cells. C, Nuclear β‐catenin expression in the indicated cells. Lamin B served as a loading control for nuclear fraction. D,E, Expression levels of TCF1, cyclin D1, Axin2 and MMP7 in the indicated cells. F, The protein expression of β‐catenin in 8 pairs of gastric cancer (GC) tissues and adjacent non‐cancerous tissues. G, Spearman's correlation analysis of the correlation between TRIM32 and β‐catenin protein levels in GC tissues. A statistically significant positive correlation between TRIM32 mRNA level and the expression of cyclin D1 (H) and MMP7 (I) in 20 GC tissues. **P < 0.01. Data represent the mean ± SD