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. 2018 Sep 19;9:2141. doi: 10.3389/fimmu.2018.02141

Figure 3.

Figure 3

C01L_F03 inhibits IFNα induced gene expression of CXCL10, IFIT2, OAS2, IRF1, and SOCS3 (A). HMECs were treated with 50, 25, 10 of C01L_F03 for 48 h and with 200 U/ml of IFNα for 4 h. RNA was isolated and subjected to qPCR analysis. Experiments were performed in 3 individual repeats, which were compared by two-way ANOVA test and unpaired two-tailed student T-test. C01L_F03 inhibits IFNα stimulated binding of STAT1, STAT2 and STAT3 to the ISRE of OAS2, and GAS of IRF1 and SOCS3, and LPS stimulated binding of p65 to the NF-κB binding site of IER3 and IRF1 (B). HMECs were treated with 50 μM of C01L_F03 for 48 h. Chromatin was isolated and subjected to IP with antibodies against pSTAT1, pSTAT2, pSTAT3 or NF-κB (p65), followed by qPCR analysis (primers are listed in Table 1). Experiments were performed in 2 individual repeats, which were compared by two-way ANOVA test and unpaired two-tailed student T-test.