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. 2018 Sep 19;9:2141. doi: 10.3389/fimmu.2018.02141

Figure 4.

Figure 4

Top-scored binding conformations of STATTIC and STX-0119 in the SH2 domain of STAT1, STAT2, and STAT3 (A). STATTIC and STX-0119 compounds are in stick representation and colored according to the atomic structure. pTyr-linker is represented by green lines with pink pTyr residue. SH2 domains of STAT1, 2 and 3 in the surface representation are colored based on the distribution of the APBS electrostatic surface potential (43). Positively charged regions are indicated in blue and negatively charged regions in red. Docking simulations were performed using Surflex-Dock 2.6 program (19, 20). STATTIC and STX-0119 inhibit IFNα induced phosphorylation of STAT1, STAT2 and STAT3 (B). HMECs were treated with 10, 5, 2.5 μM of STATTIC for 8 h or with 25, 12.5, 6.25 μM of STX-0119 for 24 h and with 200 U/ml of IFNα for 1 h. Protein extracts were collected and levels of pSTAT1, pSTAT2, pSTAT3, tSTAT1, tSTAT2, tSTAT3 and α-tubulin were assessed by western blotting. Western quantification (C). Bars represent mean quantification form 3 individual repeats ± SEM as error bars.