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. 2018 Sep 19;9:2073. doi: 10.3389/fimmu.2018.02073

Figure 3.

Figure 3

IDO1 has a critical role in anti-T. gondii response in various human cells. (A) Quantitative RT-PCR analysis of IDO1 mRNA level in HFFs, Huh7 or HeLa cells that were untreated or treated with IFN-γ for 24 h, and then infected with or without T. gondii was performed. (B) WT or IDO1-KO HFFs, Huh7 or Hela cells were untreated or treated with IFN-γ for 24 h, and then lysates were detected by Western blot. (C) WT or IDO1-KO HFFs, Huh7 or Hela cells were untreated or treated with IFN-γ for 24 h, and then infected with T. gondii. The parasite survival rate after 24 h post infection was measured by luciferase assay. Western blot image is representative of three independent experiments (B). Indicated values are means of ± s.d. (three biological replicates per group from three independent experiments) (A,C). ***p < 0.001, **p < 0.01; N.S., not significant; (Student's t-test). N.D., not detected.