Table 3.
Effects of forage type (F) and incubation time (h) on bacterial colonization of Lolium perenne, Lotus corniculatus and Trifolium pratense after in vitro rumen incubation.
| Genus |
P-value |
SED |
||||
|---|---|---|---|---|---|---|
| F | T | F∗T | F | T | F∗T | |
| Acholeplasma | NS | 0.01 | NS | 0.02 | 0.04 | 0.07 |
| Achromobacter | NS | NS | NS | 0.22 | 0.38 | 0.65 |
| Acidovorax | NS | NS | NS | 0.07 | 0.12 | 0.20 |
| Acinetobacter | NS | <0.001 | NS | 0.31 | 0.53 | 0.92 |
| Agrococcus | NS | NS | NS | 0.06 | 0.10 | 0.17 |
| Alcaligenes | NS | NS | NS | 0.01 | 0.02 | 0.04 |
| Alloprevotella | NS | 0.013 | NS | 0.01 | 0.01 | 0.02 |
| Amnibacterium | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Anaerobacterium | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Anaerofustis | NS | 0.012 | 0.045 | 0.00 | 0.01 | 0.01 |
| Anaeroplasma | NS | <0.001 | NS | 0.39 | 0.67 | 1.16 |
| Anaerorhabdus | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Anaerovibrio | NS | 0.013 | 0.008 | 0.02 | 0.04 | 0.07 |
| Anaerovorax | NS | <0.001 | NS | 0.03 | 0.05 | 0.08 |
| Aneurinibacillus | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Arthrobacter | 0.046 | NS | NS | 0.01 | 0.01 | 0.02 |
| Atopobium | NS | 0.008 | NS | 0.01 | 0.02 | 0.03 |
| Bacillus | NS | NS | NS | 0.43 | 0.74 | 1.27 |
| Blautia | NS | NS | NS | 0.06 | 0.10 | 0.17 |
| Brachybacterium | NS | NS | NS | 0.01 | 0.02 | 0.04 |
| Brevibacillus | NS | NS | NS | 0.02 | 0.03 | 0.05 |
| Brevundimonas | NS | NS | NS | 0.05 | 0.08 | 0.14 |
| Bulleidia | NS | NS | NS | 0.07 | 0.12 | 0.21 |
| Burkholderia | 0.041 | 0.041 | 0.029 | 0.01 | 0.01 | 0.02 |
| Butyrivibrio | 0.001 | 0.011 | NS | 0.26 | 0.45 | 0.78 |
| Chryseobacterium | NS | 0.005 | <0.001 | 0.03 | 0.06 | 0.10 |
| Cloacibacterium | NS | NS | NS | 0.21 | 0.36 | 0.63 |
| Coprococcus | NS | <0.001 | 0.021 | 0.02 | 0.04 | 0.06 |
| Corynebacterium | NS | <0.001 | 0.034 | 0.22 | 0.38 | 0.66 |
| Curtobacterium | NS | NS | NS | 0.00 | 0.00 | 0.01 |
| Delftia | 0.002 | <0.001 | <0.001 | 0.56 | 0.97 | 1.68 |
| Denitrobacterium | NS | 0.032 | NS | 0.01 | 0.02 | 0.04 |
| Diaphorobacter | NS | NS | NS | 0.05 | 0.08 | 0.14 |
| Eubacterium | NS | 0.04 | NS | 0.00 | 0.01 | 0.01 |
| Exiguobacterium | NS | NS | NS | 0.01 | 0.02 | 0.04 |
| Ezakiella | NS | NS | NS | 0.04 | 0.06 | 0.11 |
| Facklamia | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Fibrobacter | NS | NS | NS | 0.01 | 0.03 | 0.04 |
| Kandleria | NS | 0.03 | NS | 0.00 | 0.01 | 0.01 |
| Kocuria | NS | NS | NS | 0.19 | 0.33 | 0.57 |
| Lachnobacterium | NS | <0.001 | NS | 0.67 | 1.17 | 2.02 |
| Lactobacillus | NS | <0.001 | 0.007 | 0.34 | 0.59 | 1.01 |
| Lactococcus | NS | NS | NS | 0.46 | 0.79 | 1.37 |
| Leptotrichia | NS | NS | NS | 0.09 | 0.16 | 0.27 |
| Leuconostoc | NS | NS | NS | 0.00 | 0.00 | 0.01 |
| Limnobacter | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Lysinibacillus | NS | NS | NS | 0.03 | 0.05 | 0.09 |
| Massilia | 0.018 | NS | 0.024 | 0.10 | 0.18 | 0.31 |
| Methylobacterium | NS | NS | NS | 0.00 | 0.00 | 0.01 |
| Microbacterium | NS | <0.001 | NS | 0.47 | 0.81 | 1.40 |
| Mitsuokella | NS | NS | NS | 0.08 | 0.14 | 0.25 |
| Mogibacterium | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Moryella | NS | <0.001 | NS | 0.01 | 0.02 | 0.03 |
| Mycobacterium | NS | 0.043 | NS | 0.01 | 0.02 | 0.04 |
| Nocardiopsis | NS | NS | NS | 0.00 | 0.00 | 0.01 |
| Noviherbaspirillum | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Ochrobactrum | NS | 0.001 | NS | 0.13 | 0.23 | 0.40 |
| Oligosphaera | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Olsenella | NS | <0.001 | NS | 0.33 | 0.56 | 0.98 |
| Oscillibacter | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Paenibacillus | NS | NS | NS | 0.05 | 0.09 | 0.15 |
| Paraprevotella | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Pelomonas | NS | <0.001 | NS | 0.26 | 0.45 | 0.78 |
| Prevotella | NS | <0.001 | NS | 1.50 | 2.60 | 4.51 |
| Propionibacterium | NS | <0.001 | NS | 0.20 | 0.35 | 0.61 |
| Pseudobutyrivibrio | 0.028 | <0.001 | NS | 1.30 | 2.25 | 3.90 |
| Pseudoflavonifractor | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Pseudomonas | NS | 0.009 | NS | 0.16 | 0.27 | 0.47 |
| Ralstonia | 0.048 | <0.001 | 0.003 | 0.99 | 1.71 | 2.97 |
| Rhodococcus | NS | NS | NS | 0.52 | 0.91 | 1.57 |
| Roseburia | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Rothia | NS | NS | NS | 0.07 | 0.12 | 0.20 |
| Ruminococcus | 0.017 | <0.001 | 0.047 | 0.25 | 0.44 | 0.76 |
| Saccharofermentans | NS | 0.007 | NS | 0.01 | 0.02 | 0.03 |
| Selenomonas | <0.001 | <0.001 | 0.002 | 0.08 | 0.14 | 0.24 |
| Slackia | NS | <0.001 | NS | 0.01 | 0.01 | 0.02 |
| Sphaerochaeta | NS | <0.001 | NS | 0.02 | 0.03 | 0.05 |
| Sphingomonas | 0.031 | NS | 0.026 | 0.06 | 0.10 | 0.17 |
| Staphylococcus | NS | NS | NS | 0.39 | 0.67 | 1.16 |
| Stenotrophomonas | NS | <0.001 | 0.007 | 0.35 | 0.61 | 1.05 |
| Streptococcus | NS | NS | NS | 0.09 | 0.15 | 0.26 |
| Streptophyta | 0.005 | NS | NS | 0.07 | 0.12 | 0.20 |
| Succiniclasticum | NS | 0.007 | NS | 0.01 | 0.02 | 0.03 |
| Succinivibrio | NS | NS | NS | 0.02 | 0.04 | 0.07 |
| Thermoactinomyces | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| Treponema | NS | <0.001 | NS | 0.01 | 0.01 | 0.02 |
| Turicibacter | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| Weissella | NS | 0.012 | NS | 0.02 | 0.04 | 0.07 |
| Williamsia | NS | NS | NS | 0.00 | 0.01 | 0.01 |
| unclassified | 0.02 | <0.001 | NS | 1.83 | 3.17 | 5.48 |
| u.Acidaminococcaceae | NS | NS | NS | 0.00 | 0.00 | 0.01 |
| u.Bacillaceae | NS | NS | NS | 0.10 | 0.17 | 0.29 |
| u.Comamonadaceae | 0.033 | 0.002 | <0.001 | 0.02 | 0.03 | 0.05 |
| u.Coriobacteriaceae | NS | 0.011 | NS | 0.07 | 0.12 | 0.21 |
| u.Desulfovibrionaceae | NS | <0.001 | 0.002 | 0.01 | 0.02 | 0.04 |
| u.Enterobacteriaceae | NS | 0.009 | NS | 0.84 | 1.46 | 2.53 |
| u.Erysipelotrichaceae | 0.043 | 0.006 | 0.011 | 0.01 | 0.02 | 0.03 |
| u.Eubacteriaceae | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| u.Flavobacteriaceae | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| u.Lachnospiraceae | NS | <0.001 | NS | 1.38 | 2.39 | 4.14 |
| u.Neisseriaceae | NS | NS | NS | 0.08 | 0.15 | 0.25 |
| u.Peptococcaceae | NS | 0.008 | 0.036 | 0.01 | 0.01 | 0.02 |
| u.Planctomycetaceae | NS | 0.026 | NS | 0.02 | 0.03 | 0.05 |
| u.Planococcaceae | NS | 0.013 | NS | 0.04 | 0.07 | 0.12 |
| u.Porphyromonadaceae | 0.039 | <0.001 | NS | 0.15 | 0.26 | 0.45 |
| u.Prevotellaceae | NS | <0.001 | NS | 0.18 | 0.30 | 0.53 |
| u.Rhodospirillaceae | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| u.Ruminococcaceae | NS | <0.001 | NS | 0.78 | 1.36 | 2.35 |
| u.Sutterellaceae | NS | NS | NS | 0.01 | 0.01 | 0.02 |
| u.Veillonellaceae | 0.049 | <0.001 | NS | 0.78 | 1.36 | 2.35 |
F∗T shows if there are interactions between F and T. P-values were obtained using a one-way ANOVA and SED is standard error of the difference. Note that assignment to genus was based of 97% similarity but the prefix u. signifies that the genus could not be classified with 85% confidence so family level of classification is shown. NS is shown when data is non significant (P > 0.05).