Table 1.

Primer sequences used for gene isolation, chromosomal localization, RT qPCR analysis, and TILLING.

Primers pairs		Sequence 5′−3′		Target and application	Amplicon size on gene sequence (bp)
Forward	Reverse	Forward	Reverse
A1–9F	A1–4R	TGTTGGGCCTCCTGATCG	AGCCTCAGATGCAGGGTAC	TdHsp26-A1 isolation and chromosomal localization	1,171
A2–24F	A2–18R	CCACCAGACAATCACTGCAA	CAGGGTACAGTCTCACACG	TdHsp26-A2 isolation and chromosomal localization	939
A3–20F	A3–29R	GGCGAAGATCTGCCAAAGTAT	AACCAGCACAACCCTCTA	TdHsp26-A3 isolation and chromosomal localization	1,106
B1–5F	B1–6R	GACACTCTCTCGTTTCAATTCTC	GTTATCAGCTTCTTCCGGG	TdHsp26-B1 isolation	1,182
B1–17F	B1–6R	TCTCCAACCAGGTACGCC	GTTATCAGCTTCTTCCGGG	TdHsp26-B1 1st round HRM	1,378
B1–PT10F	B1–PT10R	CGATGCGGCAGATGCTT	TGACGAGCGCGTCGC	TdHsp26-B1 2nd round HRM, chromosomal localization and RT qPCR analysis	211
A1–PT31F	A1–PT31R	CCAGGCCCAGAACGCT	CCTCCTTcTCGTCCTCCATa	RT qPCR	338
A2–PT24F	A2–PT25R	CGCTaTaGTCAGCCGCCTt	GCGaCGCTGGACTGCa	RT qPCR	206
A3–PT27F	A3–PT27R	ATGGCTGcaGCGAACGCt	CGTCGACGGAGTTGTCCCTa	RT qPCR	172
ACT–Fw	ACT–Rev	TCCTGTGTTGCTGACTGAGG	GGTCCAAACGAAGGATAGCA	Actin housekeeping	240