Table 5.
KASP marker analysis of 13 selected TdHsp26 mutant lines.
| Mutants line | Mutation | Predicted zygosity | No. plants | Confirmed zygosity | Primer pairs used for the KASP Assay | ||
|---|---|---|---|---|---|---|---|
| Hom* | Het& | WT$ | |||||
| Cham1 W4-0844 | G536A | Hom | 15 | 15 | 0 | 0 | C0844-Fw1/C0844-Fw2/C0844_1771-Rev |
| Cham1 W4-1771 | C550T | Hom | 11 | 11 | 0 | 0 | C1771-Fw1/C1771-Fw2/C0844_1771-Rev |
| Cham1 W4-0181 | G483A | Hom | 13 | 13 | 0 | 0 | C0181-Fw/C0181-Rev1/C0181-Rev2 |
| Kronos0866 | G398A | Hom | 15 | 15 | 0 | 0 | K0866-Fw1/K0866-Fw2/K0866-Rev |
| Kronos1308 | G444A | Hom | 13 | 13 | 0 | 0 | K1308-Fw1/K1308-Fw2/K1308_0265-Rev |
| Kronos0265 | C439T | Hom | 15 | 15 | 0 | 0 | K0265-Fw1/K0265-Fw2/K1308_0265-Rev |
| Kronos2202 | C361T | Hom | 16 | 13 | 0 | 0 | K2202-Fw/K2202-Rev1/K2202-Rev2 |
| Kronos0670 | C253T | Het | 16 | 3 | 8 | 5 | K0670-Fw1/K0670-Fw2/K0670-Rev |
| Kronos2205 | G460A | Hom | 15 | 15 | 0 | 0 | K2205-Fw/K2205-Rev1/K2205-Rev2 |
| Kronos2206 | C778T | Het | 15 | 6 | 3 | 6 | K2206-Fw/K2206-Rev1/K2206-Rev2 |
| Kronos2006 | C796T | Het | 11 | 3 | 3 | 5 | K2006-Fw1/K2006-Fw2/K2006-Rev |
| Kronos0869 | G616A | Hom | 16 | 15 | 0 | 0 | K0869-Fw1/K0869-Fw2/K0869-Rev |
| Kronos0367 | C425T | Hom | 15 | 15 | 0 | 0 | K0367-Fw1/K0367-Fw2/K0367-Rev |
Between 11 and 16 individuals in the M3 (cv. Cham1) or M4 (cv. Kronos) generation were sampled. The zygosity predicted with the TILLING online resource and the zygosity verified in vivo in each plant of the mutant lines is indicated.
*
Homozygous;
&
Heterozygous;
$
Wild Type.