Figure 1.

LRP6 regulates the muscle‐specific splicing of integrin‐β1D during the development of striated muscle. (A) Time‐lapse microscopy of C2C12 myoblasts throughout the proliferation and myodifferentiation phases. GM: growth medium; DM: differentiation medium. Scale bar: 10 μm. Quantitative PCR (B) and Western blotting (C) analysis of LRP6 and myodifferentiation markers at indicated time points. C‐left: typical blots; C‐right: pooled data. *P < .05 compared with Ctrl, ^# P < .05 compared with groups other than Ctrl. (E) Measurement of integrin‐β1 transcripts and proteins in C2C12 cells. Upper: examination of integrin‐β1 transcripts during myodifferentiation using gel electrophoresis; Middle and Lower: Western blotting examination of integrin‐β1 (ITGB1) and ITGB1D during myodifferentiation (middle) and under Lrp6‐deficient conditions (lower). E‐middle and ‐lower left: typical blots; E‐middle and ‐lower right: pooled data. *P < .05 compared with Ctrl. Representative images from five independent experiments with similar results are shown