Figure 5.

LRP6 guides the global RNA splicing regulation. (A‐C) Genome‐wide identification by RNA‐seq of Lrp6 loss‐induced alternative splicing (AS) events in cardiomyocytes (NRCM), HeLa cells and human umbilical vein endothelial cells (HUVEC). (A) Venn diagram for the differential AS events in wild type (Ctrl) and Lrp6 knockdown (KD) cells; (B) The pie chart for the percentage of different types of the differential AS events; (C) Number of total LRP6‐dependent AS events. (D) Functional categories of a conserved set of 94 genes with LRP6‐dependent AS between humans and rats. (E) The AS of genes identified by RNA‐seq was verified through RT‐PCR analysis in NRCMs. WT, wild type; KD, Lrp6 knockdown