Figure 3.

miR‐124a affected insulin secretion, proliferation as well as the expressions of Pdx‐1, Pax‐6, Insulin‐1, Ngn3 and GK in BMSCs. BMSCs were treated with PBS (Con) and 50 mg/L TSPG (Ind) for 28 d, and then the induced‐BMSCs were transfected with mock (M/Ind), miR‐124a mimics (m/Ind) and miR‐124a inhibitors (i/Ind) for 48 h, respectively. A, The production of insulin secretion was detected by radioimmunoassay in treated BMSCs. B, The expression level of miR‐124a was measured by qRT‐PCR assay (**P < .01 vs Con; ^^P < .01 vs M/Ind; ## P < .01 vs m/Ind). C, Cell viability was detected by CCK‐8 assay in treated BMSCs at 12, 24 and 48 h, respectively (*P < .05, **P < .01 vs Con; ^P < .05, ^^P < .01 vs M/Ind). D, The mRNA expression levels of Pdx‐1, Pax‐6, Insulin‐1, Ngn3 and GK were determined by qRT‐PCR assay in treated BMSCs (*P < .05, **P < .01 vs Con; ^P < .05, ^^P < .01 vs M/Ind; ##P < .01 vs m/Ind). E, The protein expression levels of Pdx‐1, Pax‐6, Insulin‐1, Ngn3 and GK were detected by Western blot assay in treated BMSCs