Figure 5.

BMSCs in combination with miR‐124a inhibited podocytes cell apoptosis mediated by HG. Podocytes cells were treated with PBS (Con) and 40 mmol/L HG (HG) for 24 h and were then co‐cultured with BMSCs, which were transfected with mock (M/HG), miR‐124a mimics (m/HG) and miR‐124a inhibitors (i/HG) for 48 h, respectively. A, Apoptosis ability was detected by flow cytometry. B, The number of apoptosis was respectively quantitatively analysed (*P < .05 or **P < .01 vs Con; ^^P < .01 vs HG; ##P < .01 i/HG vs M/HG; &P < .05 m/HG vs M/HG). C, The mRNA expression levels of apoptosis‐related genes (caspase‐3, Bax and Bcl‐2) were measured by qRT‐PCR assay, respectively (**P < .01 vs Con; ^^P < .01 vs HG; &P < .05 or &&P < .01 vs M/HG; ##P < .01 i/HG vs m/HG). D, The protein expression levels of caspase‐3, Bax and Bcl‐2 were detected by Western blot assay