Figure 6.

BMSCs in combination with miR‐124a regulated Notch1, NICD, Hes1 and Delta expressions. Podocytes cells were treated with PBS (Con) and 40 mmol/L HG (HG) for 24 h and were then co‐cultured with BMSCs, which were transfected with mock (M/HG), miR‐124a mimics (m/HG) and miR‐124a inhibitors (i/HG) for 48 h, respectively. A, The mRNA expression levels of Notch1, NICD, Hes1 and Delta were measured by qRT‐PCR assay, respectively (**P < .01 vs Con; ^P < .05 or ^^P < .01 vs HG; &P < .05 or &&P < .01 m/HG vs M/HG; ##P < .01 i/HG vs M/HG). B, The protein expression levels of Notch1, NICD, Hes1 and Delta were detected by Western blot assay