FIG 3.
Sandwich ELISA for detection of BmGPI12. (A) Schematic representation of the BmGPAC assay components. (B) Representative standard curve generated using 5 μl recombinant BmGPI12 at different protein concentrations. (C) Detection of BmGPI12 released from infected mouse RBCs. A total of 106 infected mouse RBCs were serially diluted and incubated for 6 h at 37°C, using short-term in vitro culture conditions. Culture supernatants were used in the BmGPAC assay. Three replicates were used for each condition. RLU, relative light units.