FIG 6.
BmGPAC and real-time PCR assays with blood from BALB/c mice infected with 106 B. microti-infected RBCs and detection of BmGPI12 in the plasma of 84 wild-caught Peromyscus leucopus mice. (A) BmGPI12 detection in plasma from untreated BALB/c mice versus combination therapy (atovaquone plus ELQ-334)-treated BALB/c mice. Each point represents the average of 5 mice. PB, prebleed (blood collection prior to B. microti infection). The dotted line represents the LOD. (B) CT values in real-time PCR assays using DNA isolated from plasma from untreated versus treated (atovaquone plus ELQ-334) BALB/c mice. Each point represents the average of 5 mice. The dotted line represents the CT cutoff value. (C) Correlation of IFA results and BmGPI12 levels. Plasma samples from all animals were initially evaluated with an IFA to detect anti-Babesia antibodies and were classified as IFA positive or IFA negative. Five microliters of plasma was assayed using the BmGPAC assay. Dotted lines represent the LODs.