FIG 8.

Multiple alignment of the SSU sequences of the Prototheca sp. type strains. Nucleotides identical across all displayed species are shaded in red, and those present in at least six sequences are boxed in blue. Blue and orange arrows indicate forward and reverse primers, respectively. Alignment positions boxed in gray are nucleotide coordinates for the adjacent alignment block. Additional black lines indicate sequences based on which species-specific primers were designed. Primer names are given above the arrows, and numbers in brackets correspond to numbers provided in Table S7 in the supplemental material. Dots in the alignment represent the intersequence gaps, while an ellipsis beneath the proto18S-4r-1 (#8) primer in the P. miyajii IFM 53848 SSU sequence indicates a large insert in that region.