TABLE 2.
Molecular differentiation of Prototheca species/genotypes by PCR-RFLP analysis with RsaI and TaiI and with MboI of the cytb gene fragment
| Enzyme(s) and species/genotype | Length of fragment(s) (bp)a |
|
|---|---|---|
| Determined in silico | Expected upon gel electrophoresis | |
| RsaI/TaiI | ||
| P. zopfii genotype 1 | 25, 47, 253, 319 | 50, 250, 320 |
| P. zopfii genotype 2 | 25, 47, 161, 411 | 50, 160, 410 |
| P. blaschkeae | 22, 25, 25, 61, 68, 93, 128, 222 | 60, 70, 90, 130, 220 |
| P. cutis | 25, 25, 129, 183, 282 | 130, 180, 280 |
| P. ulmea | 140, 247, 257 | 140, 250 |
| P. stagnora | 25, 140, 479 | 140, 480 |
| P. wickerhamii | 25, 25, 89, 94, 411 | 90, 400 |
| P. miyajii | 25, 619 | 620 |
| P. tumulicola | 15, 629 | 620 |
| MboI | ||
| P. miyajii | 151, 221, 272 | 150, 220, 270 |
| P. tumulicola | 644 | 640 |
a
The sizes were rounded to the nearest 10. Fragments of ca. 20 bp in length were excluded since they are hardly visible even in high-percentage (4%) agarose gels.