TABLE 2.

Targeted genes, sequences of primers and probes, and species detection by Kingella-specific NAATs

Targeted gene	Sequence (5′→3′)		Result		Reference
	Primer (forward, reverse)	Probe	K. kingae	K. negevensis
rtxA	GCGCACAAGCAGGTGTACAA, ACCTGCTGCTACTGTACCTGTTTTAG	TTGAACAAAGCTGGACACG	+	+	22
	TGCCAAAGTAAAACCAGCTGAA, AACTTACCTAATTTTGGCAAAGCAA	TGACAACAAACCGCTAATCATTTCTAAGGCC	+	+	23
rtxB	CAACATAAGCCGCCAGTTGA, ACAATTAAAGCAATGGCAGTTGAG	ATCCCAACGGCGCGTCATTTGT	+	+	23
groEL	GCAAGAAGTCGGCAAAGAG, GTCAAACAACAACACAAATGGG	CGCGATCGCGACAAGTAGCCACGGTCAAGATCGCG	+	−	4
	CACGTTCTGCATTGAAATCTG, GTTCACTACTACAGACGCTTC	CGCTGACCAAGAAGCTGGCGTG	−	+	26
	CCGATTTGAAACGCGGTATT, TTTGCCAACTTGCTCGTCAG	AGTGGCGGCTTTGGTTGGG	+	−	29
mdh	TGTTCCGCATTGCTTCTG, TCATGCCGTCCAACAATG	CATCATCACGCCCTGAACGGCTT	+	−	30