Fig. 1.

LC development requires RARα. a Frequency of langerin⁺ cells in the epidermis of WT and ∆Rara^CD11c mice. b Immunofluorescence detection of langerin⁺ DCs in cross-sections of the ear skin of WT and ∆Rara^CD11c mice. The white dashed lines indicate the border between the epidermis and dermis. c Immunofluorescence detection of langerin⁺ DCs in epidermal sheets of the ear skin of WT and ∆Rara^CD11c mice. Representative and combined data are shown. d Langerin expression by dermal DCs from WT vs. ∆Rara^CD11c mice. As WT control mice, C57BL/6 or CD11C-Cre-GFP mice were used. Representative and combined data (n = 4–8) from at least 3 experiments are shown. *Significant differences from control mice by Mann-Whitney U test (p < 0.05, unpaired, 2-sided). Error bars are defined as s.e.m