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. 2018 Sep 25;9:3896. doi: 10.1038/s41467-018-06341-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — RA and RARα reciprocally regulate the expression of the positive and negative LC-regulating transcription factors, Runx3 and C/EBPβ. a Impact of RA and RARα deficiency on Runx3 expression at mRNA level. b Impact of RA and RARα deficiency on Runx3 expression at protein level. c Effect of enforced Runx3 expression on BM-derived LC differentiation in the presence and absence of RA. The data shown are gated for transduced Thy1.1⁺CD11c⁺ cells. d Impact of RA and RARα deficiency on expression of Cebpb mRNA. e Impact of RA and RARα deficiency on expression of C/EBPβ protein. f Effect of dnC/EBPβ on BM-derived LC differentiation in the presence and absence of RA. BM cells from WT or ∆Rara^CD11c mice were cultured with GM-CSF and TGFβ1 for 5 days (3 days following retroviral transduction) in the presence of At-RA (10 nM except in panels c and f where 0.1 nM was used) in a medium containing charcoal-treated FBS. Representative and combined data (n = 3–7) from at least 3 experiments are shown. Significant differences from controls by one-way ANOVA with Bonferroni adjustments (p < 0.05)* or between indicated groups by two-way ANOVA with Tukey adjustments (p < 0.05)**. Error bars are defined as s.e.m