Fig. 6.

The DNA binding, but not ligand binding, function of RARα is required for BM-LC generation. Impact of RARα variants on BM-derived LC differentiation in vitro. BM cells were transduced with retroviral vectors harboring WT and variant RARα genes that lack ligand binding (ΔLB) or DNA binding (ΔDB) activity and differentiated with GM-CSF and TGFβ1 for 5 days in the presence of RA in medium containing charcoal-treated FBS. The data shown are for transduced Thy1.1⁺CD11c⁺ cells. Representative and combined data are shown. At-RA was used at 0.1 nM. Representative and combined data from 5 experiments are shown. *^,**Significant differences by two-way ANOVA with Tukey adjustments (p < 0.05). Error bars are defined as s.e.m