Figure 2.

lncRNA SNHG14 promotes proliferation, migration and invasion of breast cancer cells. A, The oligonucleotides labelled with GFP green fluorescence were transfected as described in Methods. B, Transfection efficiency was identified by detecting SNHG14 expression via RT‐qPCR. C, CCK8 assay showed the functional effect of SNHG14 on cell proliferation of breast cancer cells. D, Colony formation assay was used to determine the functional role of SNHG14 for colony formation. E, Immunofluorescence analysis of Ki‐67 expression in breast cancer cells after infection of respective oligonucleotides. F, FACS cell cycle analysis of cells overexpressed or knockdown of SNHG14. The distribution of the cell cycle was shown in the graphs. G,H, The effects of SNHG14 on cell migration and invasion were determined using wound‐heal assay and transwell invasion assay. *P < .05, **P < .01, ***P < .001