Figure 4.

PABPC1 is a downstream target of lncRNA SNHG14 function in breast cancer. A, Schematic diagram of network of PABPC1 based on ENCODE database (http://genome.ucsc.edu). B, PABPC1 was positively regulated by SNHG14 at both transcript and protein levels in breast cancer cells. C, PABPC1 expression was detected via Western blot after transfection of sh‐PABPC1 or Lv‐PABPC1. D, Knockdown of PABPC1 dramatically abrogated the effects of Lv‐SNHG14 on cell proliferation, whereas overexpression of PABPC1 reversed the effect induced by sh‐SNHG14. E, PABPC1 knockdown or overexpression vector reversed the Lv‐SNHG14‐ or sh‐SNHG14‐indued effects of cell invasion, respectively. F, Cells of different groups were treated with trastuzumab (7.0 mg/mL) for 48 h, then the cell viability was determined using CCK8 assay. *P < .05, **P < .01