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. 2018 Jul 31;22(10):5132–5144. doi: 10.1111/jcmm.13802

Figure 4.

Figure 4

Parkin inhibition counteracts the effects of melatonin on mitophagy. A, Protein expression with representative gel blots of Parkin, Mito‐p62, COX4, p62, LC3, GAPDH (loading control for Parkin, p62, LC3). B, Relative level of Parkin. C, Relative level of p62. D, Relative level of Mito‐p62. E, Relative level of LC3 II. (n = 4). *P < 0.05 vs Con; † P < 0.05 vs HG; ‡ P < 0.05 vs HG + Ad‐LacZ; § P < 0.05 vs HG + Ad‐sh‐Parkin; and || P < 0.05 vs HG + Mel. F, Representative cardiomyocyte images of transmission electron microscopy (TEM) (Scale bars: upper panel 1 μm, and lower panel 400 nm). G, Representative colocalization images of GFP‐LC3 (Green) and mitochondria (MitoTracker Red) (Scale bars: 20 μm). H, Quantitative analysis of GFP‐LC3 punctae per cell. I, Percentage of cells with LC3 and mitochondria (MitoTracker Red, MTR) colocalization. The columns and error bars represent the means and standard deviations (SD) (n = 30 cells). *P < 0.05 vs Con; † P < 0.05 vs HG; ‡ P < 0.05 vs HG + Ad‐LacZ; § P < 0.05 vs HG + Ad‐sh‐Parkin; and || P < 0.05 vs HG + Mel