Figure 4.

Lox inhibition together with BMP4 enhances bone formation in vivo. A, C3H10T1/2 stem cells transfected with Lox RNAi or negative RNAi in the presence of BMP4 were transplanted with HA‐TCP (hydroxyaptite‐tricalcium phosphate) subcutaneously into the armpit of immunocompromised mice for 4 wk. The transplants were harvested. Expression of osteogenic and adipogenic markers in transplants was evaluated by Q‐PCR (n = 4). B, BMP4 expression in the femur from WT and BMP4‐Tg mice. C, 3D micro‐CT images of cross sections (upper panel) and vertical sections (lower panel) of right femur extracted from BMP4‐Tg mice with PBS or BAPN treatment. Bars: upper panel, 200 μm; lower panel, 1 mm. D, Quantification of trabecular and cortical bone for the same femur as C. Results are shown as mean ± SEM. E, Western blotting of Lox, CHOP‐10, PPARγ, 422/aP2, Col1α1 and Ocn expression in the right femur extracted from BMP4‐Tg mice with PBS or BAPN treatment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001