Figure 4.

Cell death induced by PTL and DMAPT depends of ROS induction. K562 and KCL‐22 cell lines were pre‐treated with N‐acetyl cysteine (NAC) for 1 h and subsequent treatment with PTL or DMAPT at 7.5 μmol/L or 10 μmol/L, ROS levels and cell death were analysed by flow cytometry. A, Shows a representative histogram of cells pre‐treated with or without NAC and treated afterwards with PTL. B, represents MFI levels of specific ROS (Hydrogen Peroxide or Superoxide Anion) in NAC pre‐treated cells compared to those treated with PTL or DMAPT for 6 h (C) indicates the cell death index after 24 h of treatment with PTL or DMAPT in NAC pre‐treated cells compared to those treated only with PTL or DMAPT. Significance between treated cells and control in MFI of ROS levels and cell death index was determined using an unpaired Student t‐test. *P < .05 **P < .01, ***P < .001, ****P < .0001